Overview

In the post-genomic era, Life science career aspirants must equip themselves with tools and techniques for efficient storage, processing and analysis of big 'Omics’ data. Chromatin Immunoprecipitation / ChIP-Seq data analysis is one of the widely practiced exercise to study differential binding of proteins under different conditions in biological systems. Chip-seq holds many promises for studying gene regulation, such as identification of in vivo transcription factor binding sites, histone modifications etc. The knowledge of ChIP-Seq data analysis will greatly help you to meet the everyday challenges posed by your Next Generation Sequencing data and can save a lot of your time and efforts to deal with it. 

Attending ChIP-Seq Data Analysis course will enable you to learn basic concepts of ChIP-Seq along with hands-on experience on solving real biological data. Trained by experienced research professionals from various life science domains, you will be introduced with its applicability to real-time problems.

Course content

  • Software installation
    • Installation and configuration of software
    • MACS, HOMER
    • Cutadapt, IGV
    • R and Bioconductor​
  • Basics of ChIP-Seq Data analysis
    • Background of Next generation sequencing
    • Raw data and file formats
    • Quality control
    • Peak calling algorithms
    • Motif enrichment and annotation
  • Basic primers for Linux and R
  • Databases for ChIP-Seq data
  • Quality control of data
  • ChIP-Seq workflow
    • Alignment of NGS reads against reference genome using Bowtie
    • Manipulation of files using SAMtools / BEDtools
    • Peak finding using MACS algorithm
    • Data visualization and enrichment browsing using UCSC genome browser
    • Extraction of sequences from enriched region and identification of consensus motifs by HOMER / MEME
  • Case Study

Duration

  • Online training.
  • 15-20 hrs 
  • Flexible timings as per your conveniences.

Extra Benefits
  • Course certificate will be provided
  • Customized case study related to your area of interest can be designed
  • Post training, if you need any help in writing codes for solving any of your research problem, assistance and guidance will be provided.

Register for this course

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