Overview

In the post-genomic era, Life science career aspirants must equip themselves with tools and techniques for efficient storage, processing and analysis of big 'Omics’ data. Chromatin Immunoprecipitation / ChIP-Seq data analysis is one of the widely practiced exercise to study differential binding of proteins under different conditions in biological systems. Chip-seq holds many promises for studying gene regulation, such as identification of in vivo transcription factor binding sites, histone modifications etc. The knowledge of ChIP-Seq data analysis will greatly help you to meet the everyday challenges posed by your Next Generation Sequencing data and can save a lot of your time and efforts to deal with it. 

Attending ChIP-Seq Data Analysis course will enable you to learn basic concepts of ChIP-Seq along with hands-on experience on solving real biological data. Trained by experienced research professionals from various life science domains, you will be introduced with its applicability to real-time problems.

Course content

  • Software installation
    • Installation and configuration of software
    • MACS, HOMER
    • Cutadapt, IGV
    • R and Bioconductor​
  • Basics of ChIP-Seq Data analysis
    • Background of Next generation sequencing
    • Raw data and file formats
    • Quality control
    • Peak calling algorithms
    • Motif enrichment and annotation
  • Basic primers for Linux and R
  • Databases for ChIP-Seq data
  • Quality control of data
  • ChIP-Seq workflow
    • Alignment of NGS reads against reference genome using Bowtie
    • Manipulation of files using SAMtools / BEDtools
    • Peak finding using MACS algorithm
    • Data visualization and enrichment browsing using UCSC genome browser
    • Extraction of sequences from enriched region and identification of consensus motifs by HOMER / MEME
  • Case Study

Duration

Extra Benefits
  • Course certificate will be provided
  • Customized case study related to your area of interest can be designed
  • Post training, if you need any help in writing codes for solving any of your research problem, assistance and guidance will be provided.
  • Online training.
  • 15-20 hrs 
  • Flexible timings as per your conveniences.

Register for this course

Please fill the Google Form below to know more and register your interest in variety of the services we offer to Empower Education and Research in Life Sciences!

Google Form (Thanks Google!)​​
Picture